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Porcine pseudorabies—— Performance evaluation of different porcine pseudorabies virus gE protein ELISA antibody kits


Pseudorabies virus (PRV) is an enveloped, double-stranded DNA virus belonging to the family Herpesviridae. Also known as suid herpesvirus-1 (SuHV-1), is the causative agent of Aujeszky ’s disease, an infection of major economic impact in animal husbandry.PRV causes high morbidity and mortality, and direct oronasal contact is the main route of transmission in domestic swine.

PRV eradication program are typically based on control strategies including culling of PRV-positive herds, vaccination programs with ‘marker’ viruses, gE-deleted strains. Gene-deleted ‘marker’ vaccines allow the identification of vaccinated, PRV-uninfected animals from wildtype-infected animals.

Enzyme linked immunosorbent assay is the strong specificity and high sensitivity diagnostic approach. Testing of large volume samples with the results within 2-3h. So PRV gE ELISA antibody test kit is commonly used in clinical practice for identification pseudorabies wild strain, also the detection approach of PRV eradication program.

There are two manufacturers of porcine pseudorabies virus (PRV) gE protein ELISA antibody test kits that have been approved from authority so far, namely Putai and Keqian Biology. In addition, there are many other manufacturers of similar products on the market, including IDEXX and JUNO. The principle is generally a blocking method and the type of antibody detected is IgG. The scientific choice can be made by comparing the sensitivity, specificity, and reproducibility of the kits.

To evaluate the quality of different pseudorabies gE antibody kits, we selected the reagents produced by Luoyang Putai Biological Technology Co., Ltd (hereinafter referred to as "Putai ") for gE antibody detection, compared with the imported reagents, domestic reagent A and domestic reagent B, to comprehensively analyse the performance differences. The lower limit of detection, the detection of artificially infected animal serum series and repeatatbility were compared. The results of the evaluation are as follows.

  • 1. The lower limit of detection
Diagnostic Regentlower limitStandards analysis - sensitivity
Domestic A1:641:16>1:8
Domestic B1:81:2/

The lower limit of detection performance of the above four types of reagents in descending order is: Putai reagent > domestic A > imported > domestic B. The dilution of Putai for three quality control products reached P01 1:256, P02 1:64 and analytical sensitivity 1:16, which is significantly better than the other three reagents.

  • 2. the detection of artificially infected animal serum
Diagnostic Regent7 Days Post Challenge11 Days Post Challenge
Putai4/5 Turn Positive5/5 Turn Positive
Import0/5 Turn Positive5/5 Turn Positive
Domestic A0/5 Turn Positive4/5 Turn Positive
Domestic B0/5 Turn Positive4/5 Turn Positive

The four types of reagents mentioned above detected the serum series of artificially infected animals in descending order: Putai > imported > domestic A = domestic B. The 7 Days Post Challeng positive rate of Putai was better than the other three reagents, and the 11 Days Post Challenge positive rate was the same as the imported and better than the other two domestic reagents. In conclusion, the PUTAI reagent was clearly superior to the other three reagents.

  • 3. Repeatability
Diagnostic RegentRepeatability
Domestic A12/16
Domestic B12/16

The repeatability of the above four types of reagents was evaluated in the following order: Putai > imported > domestic A = domestic B. The repeatability consistency of Putai reagent was the highest, 15/16, which was better than the imported reagent and the other two domestic reagents.

By comparing the performance of the above four gE ELISA reagents, PUTAI reagent has significant advantages in sensitivity and reproducibility, which is significantly higher than other similar products and more suitable for the purification of pseudorabies in pigs.

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